Orai1 facilita l'angiogenesi dopo infarto miocardico attraverso il percorso di segnalazione Notch1.

BACKGROUND: Post-ischemic neovascularization is crucial for cardiac repair after myocardial infarction (MI). While Orai1-dependent store-operated calcium entry is known to support angiogenesis, its specific role after MI remains unclear. METHODS: We stimulated human umbilical vein endothelial cells (ECs) with serum from patients with ST-segment-elevation MI to analyze proangiogenic mechanisms and to mimic the post-infarct systemic environment. We performed integrative analysis, including transcriptomics, proteomics, post-MI mouse heart single-cell RNA sequencing, and immunostaining. RESULTS: ST-segment-elevation MI serum enhanced angiogenesis by upregulating VEGF (vascular endothelial grow