La stabilizzazione di ZNF468 da RNF4 aumenta la resistenza all'apoptosi e la resistenza al carboplatino nel cancro al seno tramite attivazione di trascrizione di XIAP.

BACKGROUND: Carboplatin is a commonly utilized chemotherapy agent for breast cancer treatment, yet resistance to this drug remains a significant clinical challenge OBJECTIVE: The objective of this study was to investigate the role and regulatory mechanism of zinc finger protein 468 (ZNF468) in carboplatin resistance in breast cancer. METHODS: To establish carboplatin-resistant cells, SK-BR-3 and MDA-MB-231 cells were exposed to increasing concentrations of carboplatin. ZNF468 expression was analyzed in clinical samples (carboplatin-resistant vs. sensitive patients, n=40) and carboplatin-resistant cells. Functional assays, including Cell Counting Kit-8 (CCK-8), EDU staining, flow cytometry,